EpCAM Antibody
EGP40, which is a 40-43kDa transmembrane epithelial glycoprotein, is also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P16422
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant human EpCAM fragment from extracellular domain (within amino acids 100-224) was used as the immunogen for the EpCAM antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
EGP40/8130R
Type
Recombinant
Applications
IHC-P
Purity
Protein A affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the EpCAM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This EpCAM antibody is available for research use only.
Storage Conditions
Aliquot the EpCAM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the EpCAM antibody should be determined by the researcher.
Location
Cell surface
Image Legend
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