EpCAM Antibody

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P16422

Host

Mouse

Reactivity

Human, Cat, Dog

Immunogen

Recombinant full-length human protein was used as the immunogen for the EpCAM antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

REGP40/7334

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the EpCAM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This EpCAM antibody is available for research use only.

Storage Conditions

Aliquot the EpCAM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the EpCAM antibody should be determined by the researcher.

Location

Cell surface

Image Legend

IHC staining of FFPE dog liver with EpCAM / CD326 antibody (clone rEGP40/7334) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.