Interleukin-1 beta Antibody / IL1B

Recognizes a protein of 17-31kDa, identified as interleukin 1beta. It shows no cross-reaction with interleukin 1alpha. Epitopes of mAb IL1B/463 and IL1B/4649 are different and provide a good pair for developing an ELISA. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE) . This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (Order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P01584

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human IL1B protein was used as the immunogen for the Interleukin-1 beta antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

IL1B/463

Applications

ELISA, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Interleukin-1 beta antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Interleukin-1 beta antibody is available for research use only.

Storage Conditions

Aliquot the Interleukin-1 beta antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Interleukin-1 beta antibody should be determined by the researcher.

Location

Cytoplasm, Extracellular (secreted)

Image Legend

IHC staining of FFPE human adrenal gland tissue with Interleukin-1 beta antibody (clone IL1B/463) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.