IL-18R alpha Antibody / IL18R1

Interleukin-18 (IL-18) has been identified as a molecule that induces IFN-Gamma production and enhances NK cell cytotoxicity. IL-18 receptor (IL-18R) is a type I membrane protein present in lung, leukocytes, spleen, liver, thymus, prostate, small intestine, colon, placenta and heart, and absent from brain, skeletal muscle, pancreas and kidney. IL-18R is present in Hodgkin s disease cell lines, and does not bind IL-1Alpha or IL-1beta. The association of IL-18 to IL-18R leads to activation of NFkB. At present two subunits of IL-18R have been characterized: IL-18RAlpha and IL-18Rbeta. IL-18RAlpha has been described as the ligand-binding chain and IL-18Rbeta as the signal-transduction chain.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q13478

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the IL-18R alpha antibody.

Clonality

Monoclonal

Isotype

IgG2 κ

Clone

IL18R1/7593

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the IL-18R alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This IL-18R alpha antibody is available for research use only.

Storage Conditions

Aliquot the IL-18R alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the IL-18R alpha antibody should be determined by the researcher.

Location

Membrane

Image Legend

IHC staining of FFPE human adrenal gland tissue with IL-18R alpha antibody (clone IL18R1/7593) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.