IL18R1 Antibody / Interleukin 18 receptor alpha

Interleukin-18 (IL-18) has been identified as a molecule that induces IFN-Gamma production and enhances NK cell cytotoxicity. IL-18 receptor (IL-18R) is a type I membrane protein present in lung, leukocytes, spleen, liver, thymus, prostate, small intestine, colon, placenta and heart, and absent from brain, skeletal muscle, pancreas and kidney. IL-18R is present in Hodgkin s disease cell lines, and does not bind IL-1Alpha or IL-1beta. The association of IL-18 to IL-18R leads to activation of NFkB. At present two subunits of IL-18R have been characterized: IL-18RAlpha and IL-18Rbeta. IL-18RAlpha has been described as the ligand-binding chain and IL-18Rbeta as the signal-transduction chain.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q13478

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the IL18R1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

IL18R1/7591

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the IL18R1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This IL18R1 antibody is available for research use only.

Storage Conditions

Aliquot the IL18R1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the IL18R1 antibody should be determined by the researcher.

Location

Membrane

Image Legend

IHC staining of FFPE human adrenal gland tissue with IL18R1 antibody (clone IL18R1/7591) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.