Thyroid Peroxidase Antibody / TPO
Thyroperoxidase (TPO) is a 933 amino acid, type I transmembrane glycoprotein that plays a key role in thyroid gland function and autoimmunity. It is present as a dimer on the apical surface of thyroid follicular cells. TPO functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA.Malignant thyroid tumors exhibit an anomaly in TPO resulting in lower affinity for anti-TPO. This antibody may aid in the differentiation between benign and malignant thyroid tumors.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P07202
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant fragment of human TPO (within amino acids 600-900) was used as the immunogen for the Thyroid Peroxidase antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
TPO/7088R
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Thyroid Peroxidase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Thyroid Peroxidase antibody is available for research use only.
Storage Conditions
Aliquot the Thyroid Peroxidase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Thyroid Peroxidase antibody should be determined by the researcher.
Location
Cytoplasm, Cell surface
Image Legend
Frequently Asked Questions
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