CD207 Antibody / Langerin

Langerhans cells (LCs) are a subset of immature dendritic cells (DCs) that specifically localize in the epidermis and other mucosal epithelia. Epidermal LCs possess strong immuno-stimulatory capacity and play a central role in the initiation and regulation of immune responses. Langerin (CD207) is a Ca2+-dependent, C-type lectin domain containing, type II transmembrane protein that induces epidermal LCs to differentiate into Birbeck granules (BG) . BGs are organelles with superimposing and zippering membranes that influence proper class I type antigen presentation to the circulating T cells. Human spleen, lymph node, thymus, liver, lung and heart express Langerin protein.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q9UJ71

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 50-250) from the human protein was used as the immunogen for the CD207 antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

LGRN/7428

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the CD207 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD207 antibody is available for research use only.

Storage Conditions

Aliquot the CD207 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CD207 antibody should be determined by the researcher.

Location

Cell surface, Cytoplasm

Image Legend

IHC staining of FFPE human skin tissue with Langerin antibody (clone LGRN/7428) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.