MSH6 Antibody / G/T mismatch-binding protein

MSH6 is a mismatch repair protein which is deficient in a high proportion of patients with microsatellite instability (MSI-H) . It has been suggested that the deficiencies in DNA mismatch repair protein (s) can be seen in some malignancies such as hereditary nonpolyposis colorectal cancer (HNPCC) and endometrial cancer. MSH6 expressed in all proliferating cells participate in repair of base-base mismatch, that occur during DNA replication. Loss of MSH6 expression leads to an accumulation of DNA replication errors in the proliferating cells, particularly in areas of the genome with short repetitive nucleotide sequences, a phenomenon known as microsatellite instability (MSI) . MSH6 always used as panel with MLH1, MSH2, PMS2, and may be useful to aid in identifying the most probable gene responsiblefor the MSI.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P52701

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the MSH6 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

MSH6/8338R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the MSH6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This MSH6 antibody is available for research use only.

Storage Conditions

Aliquot the MSH6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the MSH6 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human ovarian cancer tissue with MSH6 antibody (clone MSH6/8338R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.