MSH6 Antibody / GTBP

The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA (Order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P52701

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 374-540) from the human protein was used as the immunogen for the MSH6 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

MSH6/7064R

Type

Recombinant

Applications

ELISA, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the MSH6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This MSH6 antibody is available for research use only.

Storage Conditions

Aliquot the MSH6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the MSH6 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human ovarian carcinoma tissue with MSH6 antibody (clone MSH6/7064R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.