Macrophage migration inhibitory factor Antibody / MIF / GLIF

Macrophage migration inhibitory factor, known as MIF or Glycosylation inhibiting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P14174

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment of human protein was used as the immunogen for the Macrophage migration inhibitory factor antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

MIF/6283

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Macrophage migration inhibitory factor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This MIF antibody is available for research use only.

Storage Conditions

Aliquot the Macrophage migration inhibitory factor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Macrophage migration inhibitory factor antibody should be determined by the researcher.

Location

Secreted, Cytoplasm

Image Legend

IHC staining of FFPE human prostate tissue with Macrophage migration inhibitory factor antibody (clone MIF/6283) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.