PRAME Antibody / OIP-4
Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P78395
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 100-509) from the human protein was used as the immunogen for the PRAME antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
PRAME/8558R
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the PRAME antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This PRAME antibody is available for research use only.
Storage Conditions
Aliquot the PRAME antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the PRAME antibody should be determined by the researcher.
Location
Nucleus, Cytoplasm, Cell membrane
Image Legend
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