B7RP-1 Antibody / ICOSLG
This mAb recognizes a protein of 36kDa, identified as ICOS-L, also called B7RP-1 or B7-related protein 1. It is a ligand for the ICOS receptor that initiates T and B cell proliferation and cytokine secretion. ICOS-L interactions play an essential role in T cell-dependent B cell activation in peripheral lymphoid organs such as spleen and lymph nodes. ICOS-L protein is present in myeloid leukocytes, and by Northern blot there are 2.4, 3.0, and 7.0 kb transcripts in brain, heart, kidney, and liver, with lower expression in colon and thymus, and a 1.1 kb transcript in leukocytes. Tumor necrosis factor alpha (TNF Alpha), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) enhance its expression. LPS-induced up-regulation of ICOS-L is dependent on the MyD88-dependent signaling pathway.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
O75144
Host
Mouse
Reactivity
Human
Immunogen
A recombinant fragment of human ICOSLG/B7RP-1 protein (within amino acids 1-300) was used as the immunogen for the B7RP-1 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
ICOSL/7700
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the B7RP-1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This B7RP-1 antibody is available for research use only.
Storage Conditions
Aliquot the B7RP-1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the B7RP-1 antibody should be determined by the researcher.
Location
Cell Surface, Cytoplasm
Image Legend
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