OLIG2 Antibody

Olig2, a basic helix-loop-helix transcription factor, is involved in oligo-dendroglial specification. Olig2 expression has been reported in most glial tumors, such as oligodendrogliomas and astrocytomas. Although more than half of glioblastomas are positive for Olig2, expression is very weak in terms of both percentage of labeled cells and intensity. No Olig2 expression has been found in the non-glial tumors including neuroepithelial tumors, ependymomas, sub-ependymomas, medulloblastomas, and non-neuroepithelial tumors, such as CNS lymphomas, meningiomas, schwannomas, atypical teratoid/rhabdoid tumor, and haemangioblastomas. Compared to the strong staining seen in glioma samples, a weak expression is observed in non-tumoral brain tissue.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q13516

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 200-300) from the human protein was used as the immunogen for the OLIG2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

OLIG2/7366R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the OLIG2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This OLIG2 antibody is available for research use only.

Storage Conditions

Aliquot the OLIG2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the OLIG2 antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm

Image Legend

IHC staining of FFPE human brain tissue (RNA expression: 54.2 nTPM) with OLIG2 antibody (clone OLIG2/7366R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.