SOX18 Antibody

Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNAbinding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal. Sox-18 is a 384 amino acid nuclear protein that contains one HMG box DNA-binding domain and belongs to the Sox family of transcriptional regulators.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P35713

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human SOX18 protein fragment (within amino acids 50-250) was used as the immunogen for the SOX18 antibody.

Clonality

Monoclonal

Isotype

IgG2 κ

Clone

SOX18/3271

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the SOX18 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SOX18 antibody is available for research use only.

Storage Conditions

Aliquot the SOX18 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the SOX18 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human skin tissue with SOX18 antibody (clone SOX18/3271) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.