PCNA Antibody / Proliferating Cell Nuclear Antigen
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA) . It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This mAb is excellent for multiple applications.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
P12004
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human PCNA protein was used as the immunogen for the PCNA antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RPCNA/8859
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the PCNA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This PCNA antibody is available for research use only.
Storage Conditions
Aliquot the PCNA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the PCNA antibody should be determined by the researcher.
Location
Nucleus, Cytoplasm
Image Legend
Documents
Frequently Asked Questions
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