PCNA Antibody / Proliferating Cell Nuclear Antigen

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA) . It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This mAb is excellent for multiple applications.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

P12004

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human PCNA protein was used as the immunogen for the PCNA antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RPCNA/8859

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the PCNA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PCNA antibody is available for research use only.

Storage Conditions

Aliquot the PCNA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the PCNA antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm

Image Legend

Western blot testing of human A431, 293T and HepG2 cell lysate and mouse NIH 3T3 cell lysate with PCNA antibody. Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.