Recombinant PCNA Antibody / Proliferating Cell Nuclear Antigen
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA) . It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This mAb is excellent for multiple applications.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
P12004
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human PCNA protein was used as the immunogen for the recombinant PCNA antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RPCNA/8858
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant PCNA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant PCNA antibody is available for research use only.
Storage Conditions
Aliquot the recombinant PCNA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant PCNA antibody should be determined by the researcher.
Location
Nucleus, Cytoplasm
Image Legend
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
Forget-Me-NotTM EvaGreen® qPCR Master Mix (100 reactions)
GPN1 / XAB1 (DNA Repair & Protein Synthesis) (GPN1/2350), CF740 conjugate, 0.1mg/mL
