CD35 Antibody / Complement receptor 1

CD35, complement receptor 1, is a cell membrane-bound, monomeric glycoprotein on numerous cell types including erythrocytes, leukocytes, glomerular podocytes, and follicular dendritic cells. The primary function of CD35 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. CD35 antigen is found on erythrocytes, B cells, a subset of T cells, monocytes, as well as eosinophils, and neutrophils. Anti-CD35 is considered a mature B-cell marker which labels follicular dendritic reticulum cells and tumors derived from such cells such as follicular dendritic cell tumor/sarcoma.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P17927

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 600-900) from the human protein was used as the immunogen for the CD35 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

CR1/8284R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the CD35 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD35 antibody is available for research use only.

Storage Conditions

Aliquot the CD35 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CD35 antibody should be determined by the researcher.

Location

Cell surface

Image Legend

IHC staining of FFPE human tonsil tissue with CD35 antibody (clone CR1/8223R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.