Interleukin 10 Antibody / IL-10

Interleukin 10 (IL10) is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immuno-regulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immuno-regulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P22301

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human Interleukin 10 (IL10) protein was used as the immunogen for the Interleukin 10 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

IL10/8711

Applications

IHC-P

Purity

Protein A affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Interleukin 10 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Interleukin 10 antibody is available for research use only.

Storage Conditions

Aliquot the Interleukin 10 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Interleukin 10 antibody should be determined by the researcher.

Location

Cytoplasm, Secreted, Cell Surface

Image Legend

IHC staining of FFPE human prostate cancer tissue with Interleukin 10 antibody (clone IL10/8711) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.