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Prostein Antibody / SLC45A3

Human prostein is a 553 aa protein identified by cDNA library subtraction and subsequent high-throughput microarray screening of prostate cancer. Prostein has multiple transmembrane domains. Prostein has been shown to be uniquely expressed in normal and cancerous prostatic tissues. By immunohistochemistry, prostein is expressed in the vast majority of normal and malignant prostatic tissues, regardless of grade and metastatic status. No protein expression is detected in normal and malignant tissue samples representing the great majority of essential tissues and tumors. In particular, prostein is expressed in most of poorly differentiated prostatic carcinoma, including small cell prostate carcinoma. Prostein is more specific and sensitive for prostatic carcinomas than PSA and PSAP.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q96JT2

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human SLC45A3 protein was used as the immunogen for the Prostein antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

SLC45A3/7648

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Prostein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Prostein antibody is available for research use only.

Storage Conditions

Aliquot the Prostein antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Prostein antibody should be determined by the researcher.

Location

Membrane

Image Legend

IHC staining of FFPE human prostate tissue with Prostein antibody (clone SLC45A3/7648) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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