Glutamine Synthetase Antibody / GLUL

Glutamine synthetase (Gl Syn) forms a homo-octamer that serves as a catalyst for the amination of glutamic acid to form glutamine. This enzyme is a marker for astrocytes, which serve as the primary site of conversion of glutamic acid to glutamine in the brain. Induction of glutamine synthetase is seen upon astrocyte cell contact with neurons. Elevated expression of glutamine synthetase in glial cells has been shown to protect neurons from degeneration due to excess glutamate. Glutamine synthetase is also present in the liver and is involved in nitrogen homeostasis. Overexpression of glutamine synthetase has been shown in primary liver cancers, indicating a potential role for glutamine synthetase in hepatocyte transformation.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

P15104

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 200-373) from the human protein was used as the immunogen for the Glutamine Synthetase antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

GLUL/8996R

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Glutamine Synthetase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Glutamine Synthetase antibody is available for research use only.

Storage Conditions

Aliquot the Glutamine Synthetase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Glutamine Synthetase antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human brain tissue with GLUL antibody (clone GLUL/8996R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.