CD48 Antibody
Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI) -anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
P09326
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 59-178) from the human protein was used as the immunogen for the CD48 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
CD48/8601R
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the CD48 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CD48 antibody is available for research use only.
Storage Conditions
Aliquot the CD48 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the CD48 antibody should be determined by the researcher.
Location
Cell surface
Image Legend
Western blot testing of human Raji cell lysate with CD48 antibody. Expected molecular weight: 28-50 kDa depending on glycosylation level.
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