Recombinant CD48 Antibody

Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI) -anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

P09326

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the recombinant CD48 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RCD48/8676

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant CD48 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant CD48 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant CD48 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant CD48 antibody should be determined by the researcher.

Location

Cell surface

Image Legend

Western blot testing of human Raji cell lysate with recombinant CD48 antibody. Predicted molecular weight: 28-50 kDa depending on glycosylation level.