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S100A5 Antibody / S100 calcium binding protein A5

S-100A5 (S100 calcium binding protein A5) is a 92 amino acid protein that belongs to the S-100 family. Containing two EF-hand domains, S-100A5 binds calcium, zinc and copper. One subunit can simultaneously bind two calcium ions or two copper ions plus one zinc ion. Calcium and copper ions compete for the same binding sites on the S-100A5 protein.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P33763

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-92) from the human protein was used as the immunogen for the S100A5 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

S100A5/7472

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the S100A5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This S100A5 antibody is available for research use only.

Storage Conditions

Aliquot the S100A5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.nsjbio.com/tds-pdf/s100a5-antibody-s100-calcium-binding-protein-a5-s100a57472-v4727

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the S100A5 antibody should be determined by the researcher.

CAS Number

9007-83-4

Location

Nucleus, Cytoplasm

Image Legend

IHC staining of FFPE human brain tissue with S100A5 antibody (clone S100A5/7472) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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