FCGR1A Antibody / CD64

CD64/FCGR1A is a surface glycoprotein with high affinity for monomeric IgG, is expressed constitutively on monocytes and macrophages, and can be induced in neutrophils subsequent to IFN-gamma stimulation. It plays a putative role in the initiation of cell-mediated cytotoxicity. Thus far, three genes encoding four distinct CD64 transcripts have been described. CD64/FCGR1A has been shown to associate with signal transducing subunit of the high affinity IgE receptor.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P12314

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the FCGR1A antibody.

Clonality

Monoclonal

Isotype

IgG2 κ

Clone

FCGR1A/7497

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the FCGR1A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This FCGR1A antibody is available for research use only.

Storage Conditions

Aliquot the FCGR1A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the FCGR1A antibody should be determined by the researcher.

Location

Cell Membrane

Image Legend

IHC staining of FFPE human tonsil tissue with FCGR1A antibody (clone FCGR1A/7497) at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.