S100A16 Antibody

The S-100 protein family consists of a group of calcium-binding proteins that are exclusively expressed in vertebrates and exhibit cell and tissue-specific expression. The expression levels of its members differ in various pathological conditions. The extracellular functions of the S-100 family may include the ability to enhance neurite outgrowth, involvement in inflammation and motility of tumor cells. S-100A16 (S100 calcium binding protein A16), also known as AAG13 (aging-associated gene 13 protein), S100F or DT1P1A7, is a 103 amino acid nuclear and cytoplasmic protein that exists as a homodimer that binds one calcium ion per monomer. A member of the EF-hand superfamily, S-100A16 contains two EF-hand domains and is encoded by a gene that maps to human chromosome 1q21.3.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

Q96FQ6

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-103) from the human protein was used as the immunogen for the S100A16 antibody.

Clonality

Monoclonal

Isotype

IgG2 κ

Clone

S100A16/7411

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the S100A16 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This S100A16 antibody is available for research use only.

Storage Conditions

Aliquot the S100A16 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the S100A16 antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm

Image Legend

IHC staining of FFPE human colon carcinoma tissue with S100A16 antibody (clone S100A16/7411) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.