Calprotectin Antibody / MRP14 / S100A9

Calprotectin also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. It plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P06702

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-100) from the human protein was used as the immunogen for the Calprotectin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

S100A9/7552

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Calprotectin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Calprotectin antibody is available for research use only.

Storage Conditions

Aliquot the Calprotectin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Calprotectin antibody should be determined by the researcher.

Location

Cytoplasm, Extracellular (secreted), Cell Surface

Image Legend

IHC staining of FFPE human spleen tissue with Calprotectin antibody (clone S100A9/7552) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.