Podoplanin Antibody / PA2.26 antigen / Gp36

It recognizes a muco-protein of 38-43kDa, which is identified as Podoplanin (PDPN), also called Glycoprotein 36 (Gp36) . It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

Q86YL7

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment of human PDPN protein was used as the immunogen for the Podoplanin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

PDPN/8950R

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Podoplanin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Podoplanin antibody is available for research use only.

Storage Conditions

Aliquot the Podoplanin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Podoplanin antibody should be determined by the researcher.

Location

Cell surface

Image Legend

Western blot testing of human lung tissue lysate with Podoplanin antibody. Predicted molecular weight ~25 kDa but may be observed at higher molecular weights due to glycosylation.