Glycoprotein 36 Antibody / Gp36 / Podoplanin

It recognizes a muco-protein of 38-43kDa, which is identified as Podoplanin (PDPN), also called Glycoprotein 36 (Gp36) . It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA (Order BSA-free format for coating), Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q86YL7

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-150) from the human protein was used as the immunogen for the Glycoprotein 36 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

PDPN/8875R

Type

Recombinant

Applications

ELISA, IF, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Glycoprotein 36 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Glycoprotein 36 antibody is available for research use only.

Storage Conditions

Aliquot the Glycoprotein 36 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Glycoprotein 36 antibody should be determined by the researcher.

Location

Cell surface

Image Legend

IHC staining of FFPE human prostate tissue with Glycoprotein 36 antibody (clone PDPN/8875R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.