Gp36 Antibody / Glycoprotein 36 / Podoplanin

It recognizes a muco-protein of 38-43kDa, which is identified as Podoplanin (PDPN), also called Glycoprotein 36 (Gp36) . It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endotheliumas well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA (Order BSA-free format for coating), Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q86YL7

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-100) from the human protein was used as the immunogen for the Gp36 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

PDPN/7808R

Type

Recombinant

Applications

ELISA, WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Gp36 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Gp36 antibody is available for research use only.

Storage Conditions

Aliquot the Gp36 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Gp36 antibody should be determined by the researcher.

Location

Cell surface

Image Legend

Western blot testing of human lung tissue lysate with Gp36 antibody. Predicted molecular weight ~25 kDa but may be observed at higher molecular weights due to glycosylation.