Lysine-Specific Demethylase 1 Antibody / KDM1A / LSD1

This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

O60341

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 152-279) from the human protein was used as the immunogen for the Lysine-Specific Demethylase 1 antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

PCRP-KDM1A-1A10

Applications

FACS, WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Lysine-Specific Demethylase 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Lysine-Specific Demethylase 1 antibody is available for research use only.

Storage Conditions

Aliquot the Lysine-Specific Demethylase 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Lysine-Specific Demethylase 1 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human ovarian carcinoma tissue with Lysine-Specific Demethylase 1 antibody (clone PCRP-KDM1A-1A10) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.