ARID1A Antibody

The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2a) and Brg-1 (also designated SNF2b) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits, such as BAF250a (p270 or ARID1A) and BAF250b (ARID1B), are thought to play regulatory roles.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

O14497

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1900-2000) from the human protein was used as the immunogen for the ARID1A antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

ARID1A/7735

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the ARID1A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ARID1A antibody is available for research use only.

Storage Conditions

Aliquot the ARID1A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the ARID1A antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human ovarian cancer tissue with ARID1A antibody (clone ARID1A/7735) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.