ARID1A Antibody
The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2a) and Brg-1 (also designated SNF2b) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits, such as BAF250a (p270 or ARID1A) and BAF250b (ARID1B), are thought to play regulatory roles.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
O14497
Host
Mouse
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 1900-2000) from the human protein was used as the immunogen for the ARID1A antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
ARID1A/7735
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the ARID1A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ARID1A antibody is available for research use only.
Storage Conditions
Aliquot the ARID1A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the ARID1A antibody should be determined by the researcher.
Location
Nucleus
Image Legend
Frequently Asked Questions
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