LIN28A Antibody

LIN-28 is a highly conserved, RNA-binding, cytoplasmic protein. It consists of a cold shock domain and retroviral-type (CCHC) zinc finger motifs that were first identified in Caenorhabditis elegans. LIN-28 controls the timing of events during embryonic development and is readily expressed in embryos, embryonic stem cells and embryonal carcinoma cells. The presence of LIN-28 persists in some adult tissues including cardiac and skeletal muscle. In differentiating myoblasts, LIN-28 increases protein synthesis efficiency and binds to the growth and differentiation factor IGF-II.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells

UniProt

Q9H9Z2

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 134-187) from the human protein was used as the immunogen for the LIN28A antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

PCRP-LIN28A-1E2

Applications

FACS

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the LIN28A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This LIN28A antibody is available for research use only.

Storage Conditions

Aliquot the LIN28A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the LIN28A antibody should be determined by the researcher.

Location

Nucleus

Image Legend

Flow cytometry testing of PFA-fixed human HeLa cells with LIN28A antibody (clone PCRP-LIN28A-1E2) followed by goat anti-mouse IgG-CF488 (blue), Red = unstained cells.