Recombinant PDPN Antibody / Podoplanin / Gp36

It recognizes a muco-protein of 38-43kDa, which is identified as Podoplanin (PDPN) . It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

Q86YL7

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human Podoplanin protein fragment (within amino acids 1-100) was used as the immunogen for the recombinant PDPN antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RPDPN/6994

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant PDPN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant PDPN antibody is available for research use only.

Storage Conditions

Aliquot the recombinant PDPN antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant PDPN antibody should be determined by the researcher.

Location

Cell Surface, Cytoplasm

Image Legend

IHC staining of FFPE human tonsil tissue with recombinant PDPN antibody (clone rPDPN/6994) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.