Mitotic centromere associated kinesin Antibody / MCAK / KIF2C

Kinesin family member 2c (KIF2C), alternately known as mitotic centromere associated kinesin (MCAK), is a member of the kinesin-like family of proteins. KIF2C is a cytoplasmic and nuclear protein, present throughout the cell cycle. KIF2C associates with the centromere early in prophase, and disassociates after telophase. KIF2C is abundant in thymus and testis, and present at lower levels in small intestine, the mucosal lining of the colon, and placenta. Human KIF2C maps to chromosome 1p34.1.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q99661

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 500-700) from the human protein was used as the immunogen for the Mitotic centromere associated kinesin antibody.

Clonality

Monoclonal

Isotype

IgG2c κ

Clone

KIF2C/6523

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Mitotic centromere associated kinesin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Mitotic centromere associated kinesin antibody is available for research use only.

Storage Conditions

Aliquot the Mitotic centromere associated kinesin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Mitotic centromere associated kinesin antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

IHC staining of FFPE human testis tissue with Mitotic centromere associated kinesin antibody (clone KIF2C/6523) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.