GLCLR Antibody / GCLM

G-glutamylcysteine synthetase (g-GCS) is the rate limiting enzyme for glutathione (L-g-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. g-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. The human g-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human g-GCS gene encoding the regulatory subunit maps to chromosome 1p22.1. The two subunits of g-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P48507

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human GLCLR protein was used as the immunogen for the GCLM antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

GCLM/4069

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the GLCLR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This GLCLR antibody is available for research use only.

Storage Conditions

Aliquot the GLCLR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the GLCLR antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human ovarian cancer tissue with GLCLR antibody (clone GCLM/4069) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.