Myeloid zinc finger 1 Antibody / MZF1

Zinc finger genes that encode metal-binding proteins are transcriptional regulators of other genes. Myeloid zinc finger 1 (MZF-1), also designated zinc finger protein 42, and transcription factor ZBP-89, also designated zinc finger protein 148, belong to the Kruppel C2H2-type zinc-finger protein family. The gene encoding for the MZF-1 protein maps to chromosome 19q13.43 while the gene encoding for ZBP-89 is localized on chromosome 3q21. These proteins are nuclear proteins involved in the regulation of transcriptional events. MZF-1 regulates transcription during hemopoietic development and plays a role in myeloid cell differentiation. MZF-1 regulates the CD34 promoter in a tissue-specific manner. MZF-1 and FHL3 can form a complex of high molecular mass with other proteins in the nucleus. It is induced by retinoic acid and is primarily expressed in differentiating myeloid cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P28698

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 37-128) from the human protein was used as the immunogen for the Myeloid zinc finger 1 antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

PCRP-MZF1-1E8

Applications

FACS, IF, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Myeloid zinc finger 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Myeloid zinc finger 1 antibody is available for research use only.

Storage Conditions

Aliquot the Myeloid zinc finger 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Myeloid zinc finger 1 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human colon carcinoma tissue with Myeloid zinc finger 1 antibody (clone PCRP-MZF1-1E8) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.