Recombinant Bax Antibody
Recognizes a protein of 21kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1-2 µg/mL
UniProt
Q07812
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 1-100 from human BAX protein was used as the immunogen for the recombinant Bax antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
BAX/8960R
Type
Recombinant
Applications
WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant Bax antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant Bax antibody is available for research use only.
Storage Conditions
Aliquot the recombinant Bax antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant Bax antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Western blot testing of human A549 cell lysate with recombinant Bax antibody (clone BAX/8960R) . Predicted molecular weight ~21 kDa.
Documents
Frequently Asked Questions
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