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Recombinant NAPSA Antibody / Napsin A

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

O96009

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 50-150) from the human protein was used as the immunogen for the recombinant NAPSA antibody antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RNAPSA/7239

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant NAPSA antibody antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant NAPSA antibody is available for research use only.

Storage Conditions

Aliquot the recombinant NAPSA antibody antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant NAPSA antibody antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human lung adenocarcinoma tissue with recombinant NAPSA antibody antibody (clone rNAPSA/7239) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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