ERCC1 Antibody
Recognizes a protein of 110kDa, identified as Excision Repair Cross Complementing 1 (ERCC1) . It is a mammalian nucleotide excision repair (NER) enzyme involved in repair of damaged DNA. ERCC1 is a homologous to RAD10 in Saccharomyces cerevisiae, which is required in mitotic intrachromosomal recombination and repair. ERCC1 is required in repair of cisplatin-induced DNA adducts and ultraviolet (UV) -induced DNA damage. High expression of ERCC1 has been linked to tumor progression in a variety of cancers including non-small cell lung cancer (NSCLC), squamous cell carcinoma of the head, ovarian cancer and esophageal cancer.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P07992
Host
Mouse
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 191-281) from the human protein was used as the immunogen for the ERCC1 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
ERCC1/7596
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the ERCC1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ERCC1 antibody is available for research use only.
Storage Conditions
Aliquot the ERCC1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the ERCC1 antibody should be determined by the researcher.
Location
Nucleus
Image Legend
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