ERCC1 Antibody

Recognizes a protein of 110kDa, identified as Excision Repair Cross Complementing 1 (ERCC1) . It is a mammalian nucleotide excision repair (NER) enzyme involved in repair of damaged DNA. ERCC1 is a homologous to RAD10 in Saccharomyces cerevisiae, which is required in mitotic intrachromosomal recombination and repair. ERCC1 is required in repair of cisplatin-induced DNA adducts and ultraviolet (UV) -induced DNA damage. High expression of ERCC1 has been linked to tumor progression in a variety of cancers including non-small cell lung cancer (NSCLC), squamous cell carcinoma of the head, ovarian cancer and esophageal cancer.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P07992

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 191-281) from the human protein was used as the immunogen for the ERCC1 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

ERCC1/7596

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the ERCC1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ERCC1 antibody is available for research use only.

Storage Conditions

Aliquot the ERCC1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the ERCC1 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human thyroid tissue with ERCC1 antibody (clone ERCC1/7596) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.