Uroplakin 1A Antibody / UPK1A

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. The protein may also play a role in tumor suppression.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA (Order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

O00322

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 100-200) from the human protein was used as the immunogen for the Uroplakin 1A antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

UPK1A/8704R

Type

Recombinant

Applications

ELISA, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Uroplakin 1A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Uroplakin 1A antibody is available for research use only.

Storage Conditions

Aliquot the Uroplakin 1A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Uroplakin 1A antibody should be determined by the researcher.

Location

Cell Surface

Image Legend

IHC staining of FFPE human bladder carcinoma with Uroplakin 1A antibody (clone UPK1A/8704R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.