KLK5 Antibody / Kallikrein 5

Kallikreins (KLKs) belong to the serine protease family of proteolytic enzymes. Human pancreatic/renal KLK encodes for the KLK1 enzyme, which is involved in post-translational processing of polypeptide precursors. The function of the other members of KLK gene family is still currently unknown, but evidence suggests that many KLKs are implicated in carcinogenesis. The human KLK gene family consists of 15 serine proteases. The human KLK genes are clustered on chromosome 19q13. Unlike other kalllikreins, the KLK4-15 encoded proteases are less related and do not contain a conventional KLK loop. Clusters of genes exhibit high prostatic (KLK2-4, KLK15) or pancreatic (KLK6-13) expression. KLK2 is also known as glandular kallikrein 2, tissue kallikrein, or HGK-1 and KLK3 is known as prostate-specific antigen (PSA) . Both KLK2 and KLK3 have important applications in prostate cancer and breast cancer diagnostics. Many of the KLKs are regulated by steroid hormones and a few of them, specifically KLK3, KLK6 and KLK10, are known to be downregulated in breast and other cancers. KLK5 expression is abundant in skin, mammary gland and testis.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q9Y337

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment of the human protein (within amino acids 1-200) was used as the immunogen for the KLK5 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

KLK5/4761

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the KLK5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This KLK5 antibody is available for research use only.

Storage Conditions

Aliquot the KLK5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the KLK5 antibody should be determined by the researcher.

Location

Secreted

Image Legend

IHC staining of FFPE human skin tissue with KLK5 antibody (clone KLK5/4761) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.