UPK3B Antibody / Uroplakin 3B

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. The protein may also play a role in tumor suppression.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (Order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q9BT76

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 100-300) from the human protein was used as the immunogen for the UPK3B antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

UPK3B/3270

Applications

ELISA, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the UPK3B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This UPK3B antibody is available for research use only.

Storage Conditions

Aliquot the UPK3B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the UPK3B antibody should be determined by the researcher.

Location

Cell Surface

Image Legend

IHC staining of FFPE human bladder tissue with UPK3B antibody (clone UPK3B/3270) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.