CD22 Antibody / BL-CAM
Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM) . CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P20273
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the CD22 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
BLCAM/8113R
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the CD22 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CD22 antibody is available for research use only.
Storage Conditions
Aliquot the CD22 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the CD22 antibody should be determined by the researcher.
Location
Cell surface
Image Legend
IHC staining of FFPE human tonsil tissue with CD22 antibody (clone BLCAM/8113R) . Inset: PBS instead of primary antibody, secondary control.
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