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ICAM-1 Antibody / CD54

ICAM-1/CD54 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P05362

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment of the human protein was used as the immunogen for the ICAM-1 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

ICAM1/6968

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the ICAM-1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ICAM-1 antibody is available for research use only.

Storage Conditions

Aliquot the ICAM-1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the ICAM-1 antibody should be determined by the researcher.

Location

Cell Surface

Image Legend

IHC staining of FFPE human tonsil tissue with ICAM-1 antibody (clone ICAM1/6968) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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