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Recombinant CD54 Antibody / ICAM-1

ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P05362

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment corresponding to the intracellular portion of the human protein was used as the immunogen for the recombinant CD54 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

ICAM1/8677R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant CD54 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant CD54 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant CD54 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant CD54 antibody should be determined by the researcher.

Location

Cell Surface

Image Legend

IHC staining of FFPE human tonsil tissue with recombinant CD54 antibody (clone ICAM1/8677R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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