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EBI3 Antibody / Epstein-Barr virus induced 3

Epstein-Barr virus-induced gene 3 (Ebi3) is a widely expressed homolog to the interleukin IL-12 p40 subunit protein that forms a heterodimer with either IL-12 p35 or an IL-12 p35 homolog, p28, to create a new cytokine (IL-27) . IL-27 is an early product of activated antigen-presenting cells and drives rapid clonal expansion of naive but not memory CD4+ T cells.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (Order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

Q14213

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 366-466) from the human protein was used as the immunogen for the EBI3 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

EBI3/8903

Applications

ELISA, IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the EBI3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This EBI3 antibody is available for research use only.

Storage Conditions

Aliquot the EBI3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the EBI3 antibody should be determined by the researcher.

Location

Secreted

Image Legend

Western blot testing of human placental tissue lysate with EBI3 antibody (clone EBI3/8903) . Predicted molecular weight ~25 kDa but may be observed at higher molecular weights due to glycosylation.

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