CNN1 Antibody / Calponin 1
Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this mAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
P51911
Host
Mouse
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 197-297) from the human protein was used as the immunogen for the CNN1 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RCNN1/6918
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the CNN1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CNN1 antibody is available for research use only.
Storage Conditions
Aliquot the CNN1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.nsjbio.com/tds-pdf/cnn1-antibody-calponin-1-rcnn16918-v4585
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the CNN1 antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Western blot testing of human colon tissue lysate with CNN1 antibody. Predicted molecular weight ~34 kDa.
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